Dissemination
This section presents the communication and dissemination materials produced in the activities of project PulpIng.

Pulping research on News Medical Life Sciences (20/02/2023)
News Medical Life Sciences, one of the world’s leading open-access medical and life science hubs, have published an article entitled Pumpkin byproducts exhibit antioxidant and antimicrobial activity. This article was based on the peer-review paper by Leichtweis et al “Biological Activity of Pumpkin Byproducts: Antimicrobial and Antioxidant Properties” published in Molecules in 2022 https://www.mdpi.com/1420-3049/27/23/8366
Consortium Meeting

Final consortium meeting
At 14:30h of the 25th of July 2024, took place, in Bragança the final PulpIng consortium meeting.
Participants: Lillian Barros, Tânia Pires, Adriana Molina, Maria Leichtweis, João Barreira (IPB) | Spyridon Petropoulos (UTH) | Riadh Ksouri, Hanen Falleh (CBBC) | Neji Tarchoun (ISA-CM) | Liza Saher (CRAPC) | Luma Rossi Ribeiro, Oliver Schluter (ATB) | Alexandre Gonçalves, Joana Pesqueira, Ermelinda Silva (MORE Colab) | Isabel Oliveira, Antonio Nunes (DECORGEL)

Technical Meeting of the Pulping project 28th of April 2023, Bragança, Portugal
Midterm Report Discussion Meeting (27/01/2023)
At 10:00h of the 27th of Janurary 2023, took place, via Zoom, the Midterm Report Discussion Meeting of the PulpIng project, addressing the PRIMA recommendations following the evaluation of the Midterm Report
Participants: Lillian Barros, Tânia Pires (IPB) | Spyridon Petropoulos (UTH) | Luís Pinto (MORE CoLAB) | Miguel Azevedo (DECORGEL) | Oliver Schlutter, Luma Rossi (ATB) | Mostafa Hanza (BU)


Second Annual Technical Meeting (19/10/2022)
At 10:00h of the 19th of October 2022, took place, via Zoom, the Second Annual Technical Meeting of the PulpIng project.
Participants: Lillian Barros, Carla Pereira, Sandrina Heleno, Tânia Pires, Adriana Molina, Maria Leichtweis (IPB) | Spyridon Petropoulos (UTH) | Neji Tarchoun (ISA-CM), Hanen Falleh (CBBC), Najla Mezghani (NGB-ISA-CM) Wassim Saadaoui (ISA-CM) | Luís Pinto (MORE CoLAB) | Miguel Azevedo (DECORGEL) | Oliver Schlutter, Luma Rossi, Eva Landgraber (ATB) | Mostafa Hanza (BU) | Borhane Ziani (CRAPC)
First Technical Meeting (11/10/2021)
At the 10:00h of the 11th of October 2021 took place, via Zoom, the First Technical Meeting of project PulpIng.
Participants: Lillian Barros, Márcio Carocho, Carla Pereira, Sandrina Heleno, Tânia Pires, Adriana Molina, Maria Leichtweis (IPB) | Spyridon Petropoulos (UTH) | Neji Tarchoun, Hanen Falleh (CBBC) | Alexandre Gonçalves, Luís Pinto, Marta Barbosa (MORE Colab) | Miguel Azevedo, Marta Almeida (DECORGEL) | Oliver Schlüter, Luma Ribeiro (ATB) | Borhane Ziani (CRAPC) | Mostafa Hamza (BU).

Conferences, Workshops and Seminars

International conference on Sustainable Foods – Achieving the Sustainable Development Goals (ICSF)
On 24 and 25 of July, in Bragança, the final public seminar of the PulpIng project. The event called 𝗜𝗖𝗦𝗙 aimed to disseminate knowledge regarding innovative processes and the development of sustainable food products. It brought together researchers, industry professionals, and consumers to share innovative ideas in this field and to present results from their work on this area with a special focus on the sustainable food innovation in the Mediterranean and the development of sustainable vegetable pulps formulations. During these two days 135 persons of seven different countries participated on the conference. In total, 6 oral communications and 56 Panel Communications was presented, joined by 3 Keynotes and 2 Plenary lectures.
Agrothessaly in Larissa, Greece on March 9-12
University of Thessaly presented the scope of Pulping in Agrothessaly exhibition that took place in Larissa, Greece on March 9-12.


University of Thessaly hosted the participants of Erasmus Little Farmers project
Schoolchildren from Greece, Turkey, Romania and Bulgaria visited the experimental farm of the University of Thessaly in Velestino Greece, where they were familiarized with Pulping project.
Pulping project at the II PhD Pitch Day


1st Tunisian PulpIng program workshop
Workshop Tunisian PulpIng program workshop: Progress reports of Tunisian teams – Identification of problems to be solved, held in Tunisia on 4 February 2022, organized by CBBC.
Conference ValueFarm and PulpIng PRIMA projects held on 27 April 2023 in Bragança, organized by IPB


Workshop “Descubra o valor da abóbora por inteiro!” in the “Dia do Fascínio das Plantas” – held on 16 April 2024 in Bragança with participation of IPB.
Seminar National PulpIng Info-day- Accomplishment and future plan, held in Tunisia on 4 November 2023, organized by CBBC


National PulpIng Info-day
PulpIng project – Accomplishment and future plants
Tunisian PulpIng program workshop
The second Tunisian workshop entitled “Progress reports of Tunisian teams – Accomplishments and future plans” took place on January 5th, organized by partner CBBC.
The work conducted on project PulpIng by Tunisian partners was presented and discussed.


Sustainable recovery of compounds with preserving capacity from pumpkin by-products
The sustainable recovery of compounds with preserving capacity from pumpkin by-products was investigated at CIMO-IPB, as expected in WP2. For that purpose, a primary study was carried out evaluating more than 100 samples of different pumpkin genotypes, including the seeds, fibers strands, and peels, in terms of their chemical composition and bioactivity. With these studies, it was possible to select the most promising extracts for food preservation. An extraction optimization study was, then, carried out comparing two different methodologies in order to reach the more effective extraction conditions.
Quality evaluation of raw materials
The identification of carotenoids in the pulp fruit of the Tunisian, Greek, and Egyptian pumpkin cultivars is the present emphasis of CRAPC research center after the characterization of carotenoids in the Algerian pumpkin varieties. The ongoing research is related with WP1 Task 1.4 (Quality evaluation of raw materials).
We initially extracted the carotenoids from the samples of the pumpkin cultivars before characterizing by NMR 400 MHz spectrometer in CDCl3 and then identified in accordance with the elution order at 450 nm, and quantified by HPLC/UV-vis system equipped with a C18 column and β-carotene as an external standard. Mobile phase A is composed of methanol/water plus 10 mM of ammonium acetate, and mobile phase B was gradient-mode-operated ethyl acetate/methyl butanol.

Oral communication

XXVIII Encontro Nacional da Sociedade Portuguesa de Química (ENSPQ). Aveiro – Portugal (July, 2023)
The PulpIng Project participated in the XXVIII Encontro Nacional da Sociedade Portuguesa de Química, in Aveiro, Portugal. Maria Gabriela Leichtweis (PhD student) presented the oral communication “Pumpkin peel phenolic extracts: optimized extraction and potential as food preservatives”.
XXII EuroFoodChem congress in Belgrade, Serbia (June, 2023)
The PulpIng Project was disseminated at the XXII EuroFoodChem congress in Belgrade, Serbia. Maria Gabriela Leichtweis (PhD student) presented the oral communication “Sustainable use of pumpkin: characterization of the pulp and valorization of by-products in obtaining preservative extracts”



Seminar “New advances from PRIMA projects for improving Mediterranean Agro-Food value chains” in Oeiras, Portugal
Last Friday (May 18th 2023), the seminar “New advances from PRIMA projects for improving Mediterranean Agro-Food value chains” took place in Oeiras, Lisbon, Portugal. This event was significant in establishing synergies between PRIMA projects and other stakeholders, with the presence of Project Officer Mohamed Wageih. CIMO researchers actively participated in the seminar, with Dr. Lillian Barros moderating the “Innovative Agro-Food business” session, and PhD student Maria Gabriela Leichtweis delivering an oral presentation titled “Developing a Sustainable and Functionalized Pumpkin Fruit Pulp Formulation Using Biobased Preservatives: The PulpIng Project.” More information about the seminar can be found at: https://aphorticultura.pt/eventos/PrimaMedAgroFood/index.htm
8th Portuguese Young Chemists Meeting (8PYCheM) on May 18th 2023, in Vila Real, Portugal
The PulpIng Project participated in the 8th Portuguese Young Chemists Meeting (8PYCheM) on May 18th in Vila Real, Portugal. CIMO researchers effectively shared the project’s findings. Dr. Lillian Barros delivered a keynote speech titled “From nature to products: natural food ingredients applications,” while PhD student Maria Gabriela Leichtweis presented an oral communication on “Evaluation of the phenolic profile and bioactive potential of Portuguese and Algerian pumpkin by-products.” The book of abstracts can be accessed at: https://shorturl.at/optGL


IV Congresso Nacional das Escolas Superiores Agrárias. Santarém, Portugal. 03-04 November 2022
The PulpIng Project participated in the IV Congresso Nacional das Escolas Superiores Agrárias, in Santarém, Portugal. Maria Gabriela Leichtweis (PhD student) presented the oral communication “Subprodutos de abóbora como fonte de conservantes naturais para aplicação alimentar”.
XVI Encontro de química dos alimentos. Castelo Branco, Portugal. 23-26 October 2022
The PulpIng Project participated in the XVI Encontro de química dos alimentos, in Castelo Branco, Portugal. Maria Gabriela Leichtweis (PhD student) presented the oral communication “Pumpkin by-products as a source of preservative compounds for food application: valorization of industrial bioresidues towards a sustainable system”.


Chromatography helps in times of crisis, Webinar. Lisbon, Portugal. 17 December 2021
The PulpIng Project participated in the Chromatography helps in times of crisis, Webinar, in Lisbon, Portugal. Maria Gabriela Leichtweis (PhD student) presented the oral communication “Food industry by-products applied as pumpkin-based foodstuff preservatives”.
2nd Meeting of the Group of Portuguese Chemists Abroad of the Portuguese Chemical Society. Lisbon, Portugal. 10 June 2021
The PulpIng Project participated in the 2nd Meeting of the Group of Portuguese Chemists Abroad of the Portuguese Chemical Society. Lisbon, Portugal. 10 June 2021, in Lisbon, Portugal. Maria Gabriela Leichtweis (PhD student) presented the oral communication “Bioactive properties of bio-residues from food industry: pumpkin seeds, peels, and fibrous strands”.

- Hamdi, K.; Saadaoui, W.; Tarchoun, N. Valorization of squash (Cucurbita maxima Duch) Biodiversity: approaches and main results. 4th International Congress of Biochemistry and Microbiology Applied Technologies BMAT. 2021. Hammamet Sud. 05-06 November.
- Tarchoun, N.; Hamdi, K.; Saadaoui, W. 2021. Characterization and Valorization of local squash (Cucurbita maxima Duch) landraces: approaches and main results. 1st International congress SUSTAINABLE AGRICULTURE: TOOLS AND INNOVATIONS (AgriNov2021), held in Faculty of Sciences and Technics-USMS. Béni Mellal, Morocco, on 27-30th October 2021.
- Saadaoui, W.; Hamdi, K.; Tarchoun, N. Genetic relationships among local germplasm collection of Cucurbita maxima Duchesne through molecular markers and antioxidant properties analysis. The Fourth International Congress of Biochemistry & Microbiology Applied Technologies. BMAT, 5-7 November 2021 Hammamet- Tunisie.
- Neji TARCHOUN, Wasim SAADAOUI, Khawla HAMDI, Hanen FALLEH and Riadh KSOURI. Effect of seed priming on squash (Cucurbita maxima Duch.) seed germination and seedling characteristics under salt stress. 5th International Congress of Biochemistry & Microbiology Applied Technologies BMAT’2022 (4-6 November 2022).
- Hamdi, K.; Saadaoui, W.; Sestili,S. ; Palatani, C. ; Tarchoun, N. Genetic relationship between Tunisian and Italian Cucurbita maxima Duch. Germplasm. 10th International Molecular Biology and Biotechnology Congress (04-08 Oct. 2021 online, Turkey, University of Cukurova).
- Zar Kalai F., Yeddes W. Hammami M., Ben Mansour R., Falleh H., Barros L., Tarchoun N., Ksouri R. 2022. Antioxidant activity of Tunisian pumpkin by-products: a response surface methodology approach. 6ème Congrès International de Nutrition du 28 au 30 Octobre 2022. Hammamet, Tunisie.
- Tarchoun N.; Wassim SAADAOUI, Khawla HAMDI, Hanen FALLEH, Lillian BARROS, Riadh KSOURI. Assessment of salt and water stress tolerance of Tunisian Squash (Cucurbita maxima Duchesne) germplasm during the germination and early seedling growth. IV, BALKAN AGRICULTURAL CONGRESS AGRIBALKAN 2022/ 31 August – 2 September 2022. Edirne, Turkey.
- Neji TARCHOUN, Wasim SAADAOUI, Khawla HAMDI, Hanen FALLEH and Riadh KSOURI. The Physiological and Biochemical Responses of four Local Squash Landraces under Drought stress. 11th International Molecular Biology and Biotechnology Congress (10-12 Oct, 2021. online. Turkey, University of Cukurova).
- Insaf Msetra, Wassim Saadaoui and Neji Tarchoun. Assessment of water stress tolerance of squash local landraces by application of D-Mannitol during germination and growth stage. 4th Euro-Mediterranean Conference for Environmental Integration. 20-23 October 2022, Sousse-Tunisia.
- Riadh Ksouri, Hanen Falleh, Rim Ben Mansour, Feten Zar Kalai, Walid Yeddes, Majdi Hammami, Neji Tarchoun, Lilian Barros Recovery, Refinement and Stabilization of Preserving of High Added Compounds Value from Pumpkin By-Products. MESMAP – 10, 25-27 April 2024, İstanbul-Türkiye.
Poster communication








- Poster communication in 12th “AGROSYM 2021”, Bosnia and Herzegovina October 7-10, 2021
- Poster communication in 12th “AGROSYM 2021”, Bosnia and Herzegovina October 7-10, 2021_2
- Poster communication in Dare2Change 2023 in Porto
- Poster communication in Encontro Ciência 2021, 28-30 June in Lisbon
- Poster communication in Encontro Ciência 2022, 16-18 May in Lisbon
- Poster communication in Encontro Ciência 2023, 5-7 July in Aveiropdf
- Poster communication in Natural products application Health, Cosmetic and Food (on-line), 4 and 5 February 2021
- Poster communication in Royal Society of Chemistry Poster 2022 (on-line)
- Poster communication in XIII “AGROSYM 2022”, Bosnia and Herzegovina, October 6-9, 2022
- Poster communication, 2022
- Poster comunication in 7th Portuguese Young Chemists Meeting (7PYCheM), 19-21 May 2021, Bragança, Portugal
- Poster communication in 1 International Congress on Food, Nutrition & Public Health – Towards a sustainable future, Instituto Nacional de Saúde Doutor Ricardo Jorge, 17 November 2022. Lisbon, Portugal
- Poster communication in 1 International Congress on Food, Nutrition & Public Health – Towards a sustainable future, Instituto Nacional de Saúde Doutor Ricardo Jorge, 17 November 2022. Lisbon, Portugal
- Poster communication in 1st ASHO Congress “Biodiversity, Food and Medicina
- Poster communication in 1st International seminar on sustainable management of natural resources in arid and semi-arid areas, 24-25 October 2023, Naama, Algeria
- Poster communication in 6ème Edition de “International Congress on Bioche”
- Poster communication in 31ème Congrès International des Sciences Biologiqu
- Poster communication in 32ème Congrès International des Sciences Biologiqu
- Poster communication in 33rd International Congress of Biological Sciences
- Poster communication in MESMAP – 10, 25-27 April 2024, İstanbul-Türkiye
- Poster communication in Restaste, Greece, September 2023
- Poster communication in Restaste, Greece, September 2023
- Poster communication in Seminar on Valorization of the Biotechnological Research Results by Bioindustries, 4-5 October 2023, Constantine, Algeria
- Poster communication in SMACUMED International Conference on Water-Energy- Food-Ecosystem Nexus in the Mediterranean Region (WEFE 2023). Marrakesh, Morocco, 15-17 November 2023
- Poster communition in The 3rd ICONAHE – International Conference on Natural Health, 26-26 November 2023, Mostaganem, Algeria
Preservation studies and quality assessment during shelf-life
Preparation of pumpkin pulp added with the natural ingredient extracted from pumpkin residues that will be processed by high pressure (HPP) to evaluate the shelf life extension.


Sensory analysis
Pulping Project Sensory analysis, the 19th of June, 2024.